Immunohistochemical staining:Article Title: The Integrated UPR and ERAD in Oligodendrocytes Maintain Myelin Thickness in Adults by Regulating Myelin Protein Translation
Article Snippet: Immunohistochemical detection of CC1 (APC7, 1:50; EMD Biosciences, Gibbstown, 194 NJ, RRID:AB_2057371), myelin basic protein (MBP, 1:1000; Sternberger Monoclonals, 195 Berkeley, CA, RRID:AB_10120129), aspartoacylase (ASPA, 1:1000, kindly provided by Dr. 196 M.A.
Article Title: Oligodendrocyte-specific ATF4 inactivation does not influence the development of EAE
Article Snippet: Immunohistochemistry (IHC) for CC1 (APC7, 1:50; EMD Biosciences, Gibbstown, NJ, RRID:AB_2057371), myelin basic protein (MBP, 1:1000; Sternberger Monoclonals, Berkeley, CA, RRID:AB_10120129), CD3 (1:50; Santa Cruz Biotechnology, RRID:AB_627010), CD11b (1:50; Millipore, Temecula, CA, RRID:AB_92930), NeuN (1:500, Millipore, RRID:AB_2298772), phosphorylated neuro-filament-H (SMI31, 1:1000, Sternberger Monoclonals, RRID:AB_509995), non-phosphorylated neuro-filament-H (SMI32, 1:1000, Covance, San Diego, CA, USA, RRID:AB_509997), CHOP (1:100, Santa Cruz Biotechnology, RRID: AB_783507), calbindin 2 (1:400, Sigma-Aldrich, RRID:AB_476894), and aspartoacylase (ASPA, 1:1000, kindly provided by Dr. M.A.
Article Title: NF-κB Activation Accounts for the Cytoprotective Effects of PERK Activation on Oligodendrocytes during EAE
Article Snippet: Immunohistochemical detection of CC1 (APC7, 207 1:50; EMD Biosciences, Gibbstown, NJ, RRID:AB_2057371), myelin basic protein 208 10 (MBP,1:1000; Sternberger Monoclonals, Berkeley, CA, RRID:AB_10120129), CD3 (1:50; 209 Santa Cruz Biotechnology, RRID:AB_627010), CD11b (1:50; Millipore, Temecula, CA, 210 RRID:AB_92930), the active form of p65 (1:50; Millipore, RRID:AB_2178887), aspartoacylase 211 (ASPA, 1:500; Millipore, Catalog: ABN1698), Flag (1:250; Sigma, RRID: AB_1957945), 212 SMI32 (1:1000; Covance, RRID: AB_509997), glial fibrillary acidic protein (GFAP, 1:1000; 213 Covance, RRID:AB_2314542), and A20/TNFAIP3 (1:100; Santa Cruz Biotechnology, Catalog: 214 sc-166692) were performed.
Article Title: An expanded polyglutamine in ATAXIN1 results in a loss-of-function that exacerbates severity of Multiple Sclerosis in an EAE mouse model.
Article Snippet: We used the following primary and secondary antibodies for immunohistochemical detection: CC1 (APC7, 1:50; Millipore, RRID: AB_2057371), myelin basic protein (MBP,1:1000; BioLegend, RRID: AB_2616694), CD3 (1:50; BioLegend, RRID: AB_312658), NeuN (1:500; Abcam, RRID: AB_10711040), glial fibrillary acidic protein (GFAP; 1:200; Agilent Technologies, RRID: AB_10013382), Iba1 (1:200; FUJIFILM Wako’s, RRID: AB_839504), and Neurofilament H (NF-H) (1:200; BioLegend, RRID: AB_32715852).
Article Title: Activating transcription factor 6α deficiency exacerbates oligodendrocyte death and myelin damage in immune-mediated demyelinating diseases
Article Snippet: Immunohistochemical detection of CC1 (APC7, 1:50; EMD Biosciences, Gibbstown, NJ, RRID:AB_2057371), myelin basic protein (MBP, 1:1000; Sternberger Monoclonals, Berkeley, CA, RRID:AB_10120129), CD3 (1:50; Santa Cruz Biotechnology, Dallas, Texas, RRID:AB_627010), CD11b (1:50; Millipore, RRID:AB_92930), immunoglobulin-heavy-chain-binding protein (BiP, 1:100; Santa Cruz Biotechnology, RRID:AB_631616), Glial fibrillary acidic protein (GFAP, 1:1000, Covance, Princeton, NJ, RRID:AB_2314542), Iba1 (1:300, Wako Pure Chemical Industries, Ltd, Osaka, Japan; RRID:AB_2665520), and aspartoacylase (ASPA, 1:1000, kindly provided by Dr. M.A.
Article Title: NF-κB Activation Protects Oligodendrocytes against Inflammation
Article Snippet: Immunohistochemical detection of CC1 (APC7, 1:50; EMD Biosciences, RRID: AB_2057371 ), myelin basic protein (MBP,1:1000; Sternberger Monoclonals, RRID: AB_10120129 ), CD3 (1:50; Santa Cruz Biotechnology, RRID: AB_627010 ), CD11b (1:50; Millipore, RRID: AB_92930 ), the active form of p65 (1:50; Millipore, RRID: AB_2178887 ), glial fibrillary acidic protein (GFAP; 1:200; Agilent Technologies, RRID: AB_10013382 ), and aspartoacylase (ASPA, 1:1000, kindly provided by Dr. M.A.
Article Title: NF-κB Activation Protects Oligodendrocytes against Inflammation
Article Snippet: 224 Immunohistochemical detection of CC1 (APC7, 1:50; EMD Biosciences, Gibbstown, NJ, 225 RRID:AB_2057371), myelin basic protein (MBP,1:1000; Sternberger Monoclonals, Berkeley, 226 CA, RRID:AB_10120129), CD3 (1:50; Santa Cruz Biotechnology, RRID:AB_627010), CD11b 227 11 (1:50; Millipore, Temecula, CA, RRID:AB_92930), the active form of p65 (1:50; Millipore, 228 RRID:AB_2178887), glial fibiralliry acidic protein (GFAP; 1:200; Agilent Technologies, Santa 229 Clara, CA, RRID:AB_10013382) and aspartoacylase (ASPA, 1:1000, kindly provided by Dr. 230 M.A.
Western Blot:Article Title: The Integrated UPR and ERAD in Oligodendrocytes Maintain Myelin Thickness in Adults by Regulating Myelin Protein Translation
Article Snippet: Immunohistochemical detection of CC1 (APC7, 1:50; EMD Biosciences, Gibbstown, 194 NJ, RRID:AB_2057371), myelin basic protein (MBP, 1:1000; Sternberger Monoclonals, 195 Berkeley, CA, RRID:AB_10120129), aspartoacylase (ASPA, 1:1000, kindly provided by Dr. 196 M.A.
Article Title: Oligodendrocyte-specific ATF4 inactivation does not influence the development of EAE
Article Snippet: Immunohistochemistry (IHC) for CC1 (APC7, 1:50; EMD Biosciences, Gibbstown, NJ, RRID:AB_2057371), myelin basic protein (MBP, 1:1000; Sternberger Monoclonals, Berkeley, CA, RRID:AB_10120129), CD3 (1:50; Santa Cruz Biotechnology, RRID:AB_627010), CD11b (1:50; Millipore, Temecula, CA, RRID:AB_92930), NeuN (1:500, Millipore, RRID:AB_2298772), phosphorylated neuro-filament-H (SMI31, 1:1000, Sternberger Monoclonals, RRID:AB_509995), non-phosphorylated neuro-filament-H (SMI32, 1:1000, Covance, San Diego, CA, USA, RRID:AB_509997), CHOP (1:100, Santa Cruz Biotechnology, RRID: AB_783507), calbindin 2 (1:400, Sigma-Aldrich, RRID:AB_476894), and aspartoacylase (ASPA, 1:1000, kindly provided by Dr. M.A.
Article Title: NF-κB Activation Accounts for the Cytoprotective Effects of PERK Activation on Oligodendrocytes during EAE
Article Snippet: Immunohistochemical detection of CC1 (APC7, 207 1:50; EMD Biosciences, Gibbstown, NJ, RRID:AB_2057371), myelin basic protein 208 10 (MBP,1:1000; Sternberger Monoclonals, Berkeley, CA, RRID:AB_10120129), CD3 (1:50; 209 Santa Cruz Biotechnology, RRID:AB_627010), CD11b (1:50; Millipore, Temecula, CA, 210 RRID:AB_92930), the active form of p65 (1:50; Millipore, RRID:AB_2178887), aspartoacylase 211 (ASPA, 1:500; Millipore, Catalog: ABN1698), Flag (1:250; Sigma, RRID: AB_1957945), 212 SMI32 (1:1000; Covance, RRID: AB_509997), glial fibrillary acidic protein (GFAP, 1:1000; 213 Covance, RRID:AB_2314542), and A20/TNFAIP3 (1:100; Santa Cruz Biotechnology, Catalog: 214 sc-166692) were performed.
Article Title: An expanded polyglutamine in ATAXIN1 results in a loss-of-function that exacerbates severity of Multiple Sclerosis in an EAE mouse model.
Article Snippet: We used the following primary and secondary antibodies for immunohistochemical detection: CC1 (APC7, 1:50; Millipore, RRID: AB_2057371), myelin basic protein (MBP,1:1000; BioLegend, RRID: AB_2616694), CD3 (1:50; BioLegend, RRID: AB_312658), NeuN (1:500; Abcam, RRID: AB_10711040), glial fibrillary acidic protein (GFAP; 1:200; Agilent Technologies, RRID: AB_10013382), Iba1 (1:200; FUJIFILM Wako’s, RRID: AB_839504), and Neurofilament H (NF-H) (1:200; BioLegend, RRID: AB_32715852).
Article Title: Activating transcription factor 6α deficiency exacerbates oligodendrocyte death and myelin damage in immune-mediated demyelinating diseases
Article Snippet: Immunohistochemical detection of CC1 (APC7, 1:50; EMD Biosciences, Gibbstown, NJ, RRID:AB_2057371), myelin basic protein (MBP, 1:1000; Sternberger Monoclonals, Berkeley, CA, RRID:AB_10120129), CD3 (1:50; Santa Cruz Biotechnology, Dallas, Texas, RRID:AB_627010), CD11b (1:50; Millipore, RRID:AB_92930), immunoglobulin-heavy-chain-binding protein (BiP, 1:100; Santa Cruz Biotechnology, RRID:AB_631616), Glial fibrillary acidic protein (GFAP, 1:1000, Covance, Princeton, NJ, RRID:AB_2314542), Iba1 (1:300, Wako Pure Chemical Industries, Ltd, Osaka, Japan; RRID:AB_2665520), and aspartoacylase (ASPA, 1:1000, kindly provided by Dr. M.A.
Article Title: NF-κB Activation Protects Oligodendrocytes against Inflammation
Article Snippet: Immunohistochemical detection of CC1 (APC7, 1:50; EMD Biosciences, RRID: AB_2057371 ), myelin basic protein (MBP,1:1000; Sternberger Monoclonals, RRID: AB_10120129 ), CD3 (1:50; Santa Cruz Biotechnology, RRID: AB_627010 ), CD11b (1:50; Millipore, RRID: AB_92930 ), the active form of p65 (1:50; Millipore, RRID: AB_2178887 ), glial fibrillary acidic protein (GFAP; 1:200; Agilent Technologies, RRID: AB_10013382 ), and aspartoacylase (ASPA, 1:1000, kindly provided by Dr. M.A.
Article Title: NF-κB Activation Protects Oligodendrocytes against Inflammation
Article Snippet: 224 Immunohistochemical detection of CC1 (APC7, 1:50; EMD Biosciences, Gibbstown, NJ, 225 RRID:AB_2057371), myelin basic protein (MBP,1:1000; Sternberger Monoclonals, Berkeley, 226 CA, RRID:AB_10120129), CD3 (1:50; Santa Cruz Biotechnology, RRID:AB_627010), CD11b 227 11 (1:50; Millipore, Temecula, CA, RRID:AB_92930), the active form of p65 (1:50; Millipore, 228 RRID:AB_2178887), glial fibiralliry acidic protein (GFAP; 1:200; Agilent Technologies, Santa 229 Clara, CA, RRID:AB_10013382) and aspartoacylase (ASPA, 1:1000, kindly provided by Dr. 230 M.A.
Double Immunostaining:Article Title: The Integrated UPR and ERAD in Oligodendrocytes Maintain Myelin Thickness in Adults by Regulating Myelin Protein Translation
Article Snippet: Immunohistochemical detection of CC1 (APC7, 1:50; EMD Biosciences, Gibbstown, 194 NJ, RRID:AB_2057371), myelin basic protein (MBP, 1:1000; Sternberger Monoclonals, 195 Berkeley, CA, RRID:AB_10120129), aspartoacylase (ASPA, 1:1000, kindly provided by Dr. 196 M.A.
Article Title: Oligodendrocyte-specific ATF4 inactivation does not influence the development of EAE
Article Snippet: Immunohistochemistry (IHC) for CC1 (APC7, 1:50; EMD Biosciences, Gibbstown, NJ, RRID:AB_2057371), myelin basic protein (MBP, 1:1000; Sternberger Monoclonals, Berkeley, CA, RRID:AB_10120129), CD3 (1:50; Santa Cruz Biotechnology, RRID:AB_627010), CD11b (1:50; Millipore, Temecula, CA, RRID:AB_92930), NeuN (1:500, Millipore, RRID:AB_2298772), phosphorylated neuro-filament-H (SMI31, 1:1000, Sternberger Monoclonals, RRID:AB_509995), non-phosphorylated neuro-filament-H (SMI32, 1:1000, Covance, San Diego, CA, USA, RRID:AB_509997), CHOP (1:100, Santa Cruz Biotechnology, RRID: AB_783507), calbindin 2 (1:400, Sigma-Aldrich, RRID:AB_476894), and aspartoacylase (ASPA, 1:1000, kindly provided by Dr. M.A.
Article Title: NF-κB Activation Accounts for the Cytoprotective Effects of PERK Activation on Oligodendrocytes during EAE
Article Snippet: Immunohistochemical detection of CC1 (APC7, 207 1:50; EMD Biosciences, Gibbstown, NJ, RRID:AB_2057371), myelin basic protein 208 10 (MBP,1:1000; Sternberger Monoclonals, Berkeley, CA, RRID:AB_10120129), CD3 (1:50; 209 Santa Cruz Biotechnology, RRID:AB_627010), CD11b (1:50; Millipore, Temecula, CA, 210 RRID:AB_92930), the active form of p65 (1:50; Millipore, RRID:AB_2178887), aspartoacylase 211 (ASPA, 1:500; Millipore, Catalog: ABN1698), Flag (1:250; Sigma, RRID: AB_1957945), 212 SMI32 (1:1000; Covance, RRID: AB_509997), glial fibrillary acidic protein (GFAP, 1:1000; 213 Covance, RRID:AB_2314542), and A20/TNFAIP3 (1:100; Santa Cruz Biotechnology, Catalog: 214 sc-166692) were performed.
Article Title: An expanded polyglutamine in ATAXIN1 results in a loss-of-function that exacerbates severity of Multiple Sclerosis in an EAE mouse model.
Article Snippet: We used the following primary and secondary antibodies for immunohistochemical detection: CC1 (APC7, 1:50; Millipore, RRID: AB_2057371), myelin basic protein (MBP,1:1000; BioLegend, RRID: AB_2616694), CD3 (1:50; BioLegend, RRID: AB_312658), NeuN (1:500; Abcam, RRID: AB_10711040), glial fibrillary acidic protein (GFAP; 1:200; Agilent Technologies, RRID: AB_10013382), Iba1 (1:200; FUJIFILM Wako’s, RRID: AB_839504), and Neurofilament H (NF-H) (1:200; BioLegend, RRID: AB_32715852).
Article Title: Activating transcription factor 6α deficiency exacerbates oligodendrocyte death and myelin damage in immune-mediated demyelinating diseases
Article Snippet: Immunohistochemical detection of CC1 (APC7, 1:50; EMD Biosciences, Gibbstown, NJ, RRID:AB_2057371), myelin basic protein (MBP, 1:1000; Sternberger Monoclonals, Berkeley, CA, RRID:AB_10120129), CD3 (1:50; Santa Cruz Biotechnology, Dallas, Texas, RRID:AB_627010), CD11b (1:50; Millipore, RRID:AB_92930), immunoglobulin-heavy-chain-binding protein (BiP, 1:100; Santa Cruz Biotechnology, RRID:AB_631616), Glial fibrillary acidic protein (GFAP, 1:1000, Covance, Princeton, NJ, RRID:AB_2314542), Iba1 (1:300, Wako Pure Chemical Industries, Ltd, Osaka, Japan; RRID:AB_2665520), and aspartoacylase (ASPA, 1:1000, kindly provided by Dr. M.A.
Article Title: NF-κB Activation Protects Oligodendrocytes against Inflammation
Article Snippet: Immunohistochemical detection of CC1 (APC7, 1:50; EMD Biosciences, RRID: AB_2057371 ), myelin basic protein (MBP,1:1000; Sternberger Monoclonals, RRID: AB_10120129 ), CD3 (1:50; Santa Cruz Biotechnology, RRID: AB_627010 ), CD11b (1:50; Millipore, RRID: AB_92930 ), the active form of p65 (1:50; Millipore, RRID: AB_2178887 ), glial fibrillary acidic protein (GFAP; 1:200; Agilent Technologies, RRID: AB_10013382 ), and aspartoacylase (ASPA, 1:1000, kindly provided by Dr. M.A.
Article Title: NF-κB Activation Protects Oligodendrocytes against Inflammation
Article Snippet: 224 Immunohistochemical detection of CC1 (APC7, 1:50; EMD Biosciences, Gibbstown, NJ, 225 RRID:AB_2057371), myelin basic protein (MBP,1:1000; Sternberger Monoclonals, Berkeley, 226 CA, RRID:AB_10120129), CD3 (1:50; Santa Cruz Biotechnology, RRID:AB_627010), CD11b 227 11 (1:50; Millipore, Temecula, CA, RRID:AB_92930), the active form of p65 (1:50; Millipore, 228 RRID:AB_2178887), glial fibiralliry acidic protein (GFAP; 1:200; Agilent Technologies, Santa 229 Clara, CA, RRID:AB_10013382) and aspartoacylase (ASPA, 1:1000, kindly provided by Dr. 230 M.A.
Immunostaining:Article Title: The Integrated UPR and ERAD in Oligodendrocytes Maintain Myelin Thickness in Adults by Regulating Myelin Protein Translation
Article Snippet: Immunohistochemical detection of CC1 (APC7, 1:50; EMD Biosciences, Gibbstown, 194 NJ, RRID:AB_2057371), myelin basic protein (MBP, 1:1000; Sternberger Monoclonals, 195 Berkeley, CA, RRID:AB_10120129), aspartoacylase (ASPA, 1:1000, kindly provided by Dr. 196 M.A.
Article Title: Oligodendrocyte-specific ATF4 inactivation does not influence the development of EAE
Article Snippet: Immunohistochemistry (IHC) for CC1 (APC7, 1:50; EMD Biosciences, Gibbstown, NJ, RRID:AB_2057371), myelin basic protein (MBP, 1:1000; Sternberger Monoclonals, Berkeley, CA, RRID:AB_10120129), CD3 (1:50; Santa Cruz Biotechnology, RRID:AB_627010), CD11b (1:50; Millipore, Temecula, CA, RRID:AB_92930), NeuN (1:500, Millipore, RRID:AB_2298772), phosphorylated neuro-filament-H (SMI31, 1:1000, Sternberger Monoclonals, RRID:AB_509995), non-phosphorylated neuro-filament-H (SMI32, 1:1000, Covance, San Diego, CA, USA, RRID:AB_509997), CHOP (1:100, Santa Cruz Biotechnology, RRID: AB_783507), calbindin 2 (1:400, Sigma-Aldrich, RRID:AB_476894), and aspartoacylase (ASPA, 1:1000, kindly provided by Dr. M.A.
Article Title: NF-κB Activation Accounts for the Cytoprotective Effects of PERK Activation on Oligodendrocytes during EAE
Article Snippet: Immunohistochemical detection of CC1 (APC7, 207 1:50; EMD Biosciences, Gibbstown, NJ, RRID:AB_2057371), myelin basic protein 208 10 (MBP,1:1000; Sternberger Monoclonals, Berkeley, CA, RRID:AB_10120129), CD3 (1:50; 209 Santa Cruz Biotechnology, RRID:AB_627010), CD11b (1:50; Millipore, Temecula, CA, 210 RRID:AB_92930), the active form of p65 (1:50; Millipore, RRID:AB_2178887), aspartoacylase 211 (ASPA, 1:500; Millipore, Catalog: ABN1698), Flag (1:250; Sigma, RRID: AB_1957945), 212 SMI32 (1:1000; Covance, RRID: AB_509997), glial fibrillary acidic protein (GFAP, 1:1000; 213 Covance, RRID:AB_2314542), and A20/TNFAIP3 (1:100; Santa Cruz Biotechnology, Catalog: 214 sc-166692) were performed.
Article Title: An expanded polyglutamine in ATAXIN1 results in a loss-of-function that exacerbates severity of Multiple Sclerosis in an EAE mouse model.
Article Snippet: We used the following primary and secondary antibodies for immunohistochemical detection: CC1 (APC7, 1:50; Millipore, RRID: AB_2057371), myelin basic protein (MBP,1:1000; BioLegend, RRID: AB_2616694), CD3 (1:50; BioLegend, RRID: AB_312658), NeuN (1:500; Abcam, RRID: AB_10711040), glial fibrillary acidic protein (GFAP; 1:200; Agilent Technologies, RRID: AB_10013382), Iba1 (1:200; FUJIFILM Wako’s, RRID: AB_839504), and Neurofilament H (NF-H) (1:200; BioLegend, RRID: AB_32715852).
Article Title: Activating transcription factor 6α deficiency exacerbates oligodendrocyte death and myelin damage in immune-mediated demyelinating diseases
Article Snippet: Immunohistochemical detection of CC1 (APC7, 1:50; EMD Biosciences, Gibbstown, NJ, RRID:AB_2057371), myelin basic protein (MBP, 1:1000; Sternberger Monoclonals, Berkeley, CA, RRID:AB_10120129), CD3 (1:50; Santa Cruz Biotechnology, Dallas, Texas, RRID:AB_627010), CD11b (1:50; Millipore, RRID:AB_92930), immunoglobulin-heavy-chain-binding protein (BiP, 1:100; Santa Cruz Biotechnology, RRID:AB_631616), Glial fibrillary acidic protein (GFAP, 1:1000, Covance, Princeton, NJ, RRID:AB_2314542), Iba1 (1:300, Wako Pure Chemical Industries, Ltd, Osaka, Japan; RRID:AB_2665520), and aspartoacylase (ASPA, 1:1000, kindly provided by Dr. M.A.
Article Title: NF-κB Activation Protects Oligodendrocytes against Inflammation
Article Snippet: Immunohistochemical detection of CC1 (APC7, 1:50; EMD Biosciences, RRID: AB_2057371 ), myelin basic protein (MBP,1:1000; Sternberger Monoclonals, RRID: AB_10120129 ), CD3 (1:50; Santa Cruz Biotechnology, RRID: AB_627010 ), CD11b (1:50; Millipore, RRID: AB_92930 ), the active form of p65 (1:50; Millipore, RRID: AB_2178887 ), glial fibrillary acidic protein (GFAP; 1:200; Agilent Technologies, RRID: AB_10013382 ), and aspartoacylase (ASPA, 1:1000, kindly provided by Dr. M.A.
Article Title: NF-κB Activation Protects Oligodendrocytes against Inflammation
Article Snippet: 224 Immunohistochemical detection of CC1 (APC7, 1:50; EMD Biosciences, Gibbstown, NJ, 225 RRID:AB_2057371), myelin basic protein (MBP,1:1000; Sternberger Monoclonals, Berkeley, 226 CA, RRID:AB_10120129), CD3 (1:50; Santa Cruz Biotechnology, RRID:AB_627010), CD11b 227 11 (1:50; Millipore, Temecula, CA, RRID:AB_92930), the active form of p65 (1:50; Millipore, 228 RRID:AB_2178887), glial fibiralliry acidic protein (GFAP; 1:200; Agilent Technologies, Santa 229 Clara, CA, RRID:AB_10013382) and aspartoacylase (ASPA, 1:1000, kindly provided by Dr. 230 M.A.
Control:Article Title: The Integrated UPR and ERAD in Oligodendrocytes Maintain Myelin Thickness in Adults by Regulating Myelin Protein Translation
Article Snippet: Immunohistochemical detection of CC1 (APC7, 1:50; EMD Biosciences, Gibbstown, 194 NJ, RRID:AB_2057371), myelin basic protein (MBP, 1:1000; Sternberger Monoclonals, 195 Berkeley, CA, RRID:AB_10120129), aspartoacylase (ASPA, 1:1000, kindly provided by Dr. 196 M.A.
Article Title: Oligodendrocyte-specific ATF4 inactivation does not influence the development of EAE
Article Snippet: Immunohistochemistry (IHC) for CC1 (APC7, 1:50; EMD Biosciences, Gibbstown, NJ, RRID:AB_2057371), myelin basic protein (MBP, 1:1000; Sternberger Monoclonals, Berkeley, CA, RRID:AB_10120129), CD3 (1:50; Santa Cruz Biotechnology, RRID:AB_627010), CD11b (1:50; Millipore, Temecula, CA, RRID:AB_92930), NeuN (1:500, Millipore, RRID:AB_2298772), phosphorylated neuro-filament-H (SMI31, 1:1000, Sternberger Monoclonals, RRID:AB_509995), non-phosphorylated neuro-filament-H (SMI32, 1:1000, Covance, San Diego, CA, USA, RRID:AB_509997), CHOP (1:100, Santa Cruz Biotechnology, RRID: AB_783507), calbindin 2 (1:400, Sigma-Aldrich, RRID:AB_476894), and aspartoacylase (ASPA, 1:1000, kindly provided by Dr. M.A.
Article Title: NF-κB Activation Accounts for the Cytoprotective Effects of PERK Activation on Oligodendrocytes during EAE
Article Snippet: Immunohistochemical detection of CC1 (APC7, 207 1:50; EMD Biosciences, Gibbstown, NJ, RRID:AB_2057371), myelin basic protein 208 10 (MBP,1:1000; Sternberger Monoclonals, Berkeley, CA, RRID:AB_10120129), CD3 (1:50; 209 Santa Cruz Biotechnology, RRID:AB_627010), CD11b (1:50; Millipore, Temecula, CA, 210 RRID:AB_92930), the active form of p65 (1:50; Millipore, RRID:AB_2178887), aspartoacylase 211 (ASPA, 1:500; Millipore, Catalog: ABN1698), Flag (1:250; Sigma, RRID: AB_1957945), 212 SMI32 (1:1000; Covance, RRID: AB_509997), glial fibrillary acidic protein (GFAP, 1:1000; 213 Covance, RRID:AB_2314542), and A20/TNFAIP3 (1:100; Santa Cruz Biotechnology, Catalog: 214 sc-166692) were performed.
Article Title: An expanded polyglutamine in ATAXIN1 results in a loss-of-function that exacerbates severity of Multiple Sclerosis in an EAE mouse model.
Article Snippet: We used the following primary and secondary antibodies for immunohistochemical detection: CC1 (APC7, 1:50; Millipore, RRID: AB_2057371), myelin basic protein (MBP,1:1000; BioLegend, RRID: AB_2616694), CD3 (1:50; BioLegend, RRID: AB_312658), NeuN (1:500; Abcam, RRID: AB_10711040), glial fibrillary acidic protein (GFAP; 1:200; Agilent Technologies, RRID: AB_10013382), Iba1 (1:200; FUJIFILM Wako’s, RRID: AB_839504), and Neurofilament H (NF-H) (1:200; BioLegend, RRID: AB_32715852).
Article Title: Activating transcription factor 6α deficiency exacerbates oligodendrocyte death and myelin damage in immune-mediated demyelinating diseases
Article Snippet: Immunohistochemical detection of CC1 (APC7, 1:50; EMD Biosciences, Gibbstown, NJ, RRID:AB_2057371), myelin basic protein (MBP, 1:1000; Sternberger Monoclonals, Berkeley, CA, RRID:AB_10120129), CD3 (1:50; Santa Cruz Biotechnology, Dallas, Texas, RRID:AB_627010), CD11b (1:50; Millipore, RRID:AB_92930), immunoglobulin-heavy-chain-binding protein (BiP, 1:100; Santa Cruz Biotechnology, RRID:AB_631616), Glial fibrillary acidic protein (GFAP, 1:1000, Covance, Princeton, NJ, RRID:AB_2314542), Iba1 (1:300, Wako Pure Chemical Industries, Ltd, Osaka, Japan; RRID:AB_2665520), and aspartoacylase (ASPA, 1:1000, kindly provided by Dr. M.A.
Article Title: NF-κB Activation Protects Oligodendrocytes against Inflammation
Article Snippet: Immunohistochemical detection of CC1 (APC7, 1:50; EMD Biosciences, RRID: AB_2057371 ), myelin basic protein (MBP,1:1000; Sternberger Monoclonals, RRID: AB_10120129 ), CD3 (1:50; Santa Cruz Biotechnology, RRID: AB_627010 ), CD11b (1:50; Millipore, RRID: AB_92930 ), the active form of p65 (1:50; Millipore, RRID: AB_2178887 ), glial fibrillary acidic protein (GFAP; 1:200; Agilent Technologies, RRID: AB_10013382 ), and aspartoacylase (ASPA, 1:1000, kindly provided by Dr. M.A.
Article Title: NF-κB Activation Protects Oligodendrocytes against Inflammation
Article Snippet: 224 Immunohistochemical detection of CC1 (APC7, 1:50; EMD Biosciences, Gibbstown, NJ, 225 RRID:AB_2057371), myelin basic protein (MBP,1:1000; Sternberger Monoclonals, Berkeley, 226 CA, RRID:AB_10120129), CD3 (1:50; Santa Cruz Biotechnology, RRID:AB_627010), CD11b 227 11 (1:50; Millipore, Temecula, CA, RRID:AB_92930), the active form of p65 (1:50; Millipore, 228 RRID:AB_2178887), glial fibiralliry acidic protein (GFAP; 1:200; Agilent Technologies, Santa 229 Clara, CA, RRID:AB_10013382) and aspartoacylase (ASPA, 1:1000, kindly provided by Dr. 230 M.A.
Fluorescence:Article Title: The Integrated UPR and ERAD in Oligodendrocytes Maintain Myelin Thickness in Adults by Regulating Myelin Protein Translation
Article Snippet: Immunohistochemical detection of CC1 (APC7, 1:50; EMD Biosciences, Gibbstown, 194 NJ, RRID:AB_2057371), myelin basic protein (MBP, 1:1000; Sternberger Monoclonals, 195 Berkeley, CA, RRID:AB_10120129), aspartoacylase (ASPA, 1:1000, kindly provided by Dr. 196 M.A.
Article Title: Oligodendrocyte-specific ATF4 inactivation does not influence the development of EAE
Article Snippet: Immunohistochemistry (IHC) for CC1 (APC7, 1:50; EMD Biosciences, Gibbstown, NJ, RRID:AB_2057371), myelin basic protein (MBP, 1:1000; Sternberger Monoclonals, Berkeley, CA, RRID:AB_10120129), CD3 (1:50; Santa Cruz Biotechnology, RRID:AB_627010), CD11b (1:50; Millipore, Temecula, CA, RRID:AB_92930), NeuN (1:500, Millipore, RRID:AB_2298772), phosphorylated neuro-filament-H (SMI31, 1:1000, Sternberger Monoclonals, RRID:AB_509995), non-phosphorylated neuro-filament-H (SMI32, 1:1000, Covance, San Diego, CA, USA, RRID:AB_509997), CHOP (1:100, Santa Cruz Biotechnology, RRID: AB_783507), calbindin 2 (1:400, Sigma-Aldrich, RRID:AB_476894), and aspartoacylase (ASPA, 1:1000, kindly provided by Dr. M.A.
Article Title: NF-κB Activation Accounts for the Cytoprotective Effects of PERK Activation on Oligodendrocytes during EAE
Article Snippet: Immunohistochemical detection of CC1 (APC7, 207 1:50; EMD Biosciences, Gibbstown, NJ, RRID:AB_2057371), myelin basic protein 208 10 (MBP,1:1000; Sternberger Monoclonals, Berkeley, CA, RRID:AB_10120129), CD3 (1:50; 209 Santa Cruz Biotechnology, RRID:AB_627010), CD11b (1:50; Millipore, Temecula, CA, 210 RRID:AB_92930), the active form of p65 (1:50; Millipore, RRID:AB_2178887), aspartoacylase 211 (ASPA, 1:500; Millipore, Catalog: ABN1698), Flag (1:250; Sigma, RRID: AB_1957945), 212 SMI32 (1:1000; Covance, RRID: AB_509997), glial fibrillary acidic protein (GFAP, 1:1000; 213 Covance, RRID:AB_2314542), and A20/TNFAIP3 (1:100; Santa Cruz Biotechnology, Catalog: 214 sc-166692) were performed.
Article Title: An expanded polyglutamine in ATAXIN1 results in a loss-of-function that exacerbates severity of Multiple Sclerosis in an EAE mouse model.
Article Snippet: We used the following primary and secondary antibodies for immunohistochemical detection: CC1 (APC7, 1:50; Millipore, RRID: AB_2057371), myelin basic protein (MBP,1:1000; BioLegend, RRID: AB_2616694), CD3 (1:50; BioLegend, RRID: AB_312658), NeuN (1:500; Abcam, RRID: AB_10711040), glial fibrillary acidic protein (GFAP; 1:200; Agilent Technologies, RRID: AB_10013382), Iba1 (1:200; FUJIFILM Wako’s, RRID: AB_839504), and Neurofilament H (NF-H) (1:200; BioLegend, RRID: AB_32715852).
Article Title: Activating transcription factor 6α deficiency exacerbates oligodendrocyte death and myelin damage in immune-mediated demyelinating diseases
Article Snippet: Immunohistochemical detection of CC1 (APC7, 1:50; EMD Biosciences, Gibbstown, NJ, RRID:AB_2057371), myelin basic protein (MBP, 1:1000; Sternberger Monoclonals, Berkeley, CA, RRID:AB_10120129), CD3 (1:50; Santa Cruz Biotechnology, Dallas, Texas, RRID:AB_627010), CD11b (1:50; Millipore, RRID:AB_92930), immunoglobulin-heavy-chain-binding protein (BiP, 1:100; Santa Cruz Biotechnology, RRID:AB_631616), Glial fibrillary acidic protein (GFAP, 1:1000, Covance, Princeton, NJ, RRID:AB_2314542), Iba1 (1:300, Wako Pure Chemical Industries, Ltd, Osaka, Japan; RRID:AB_2665520), and aspartoacylase (ASPA, 1:1000, kindly provided by Dr. M.A.
Article Title: NF-κB Activation Protects Oligodendrocytes against Inflammation
Article Snippet: Immunohistochemical detection of CC1 (APC7, 1:50; EMD Biosciences, RRID: AB_2057371 ), myelin basic protein (MBP,1:1000; Sternberger Monoclonals, RRID: AB_10120129 ), CD3 (1:50; Santa Cruz Biotechnology, RRID: AB_627010 ), CD11b (1:50; Millipore, RRID: AB_92930 ), the active form of p65 (1:50; Millipore, RRID: AB_2178887 ), glial fibrillary acidic protein (GFAP; 1:200; Agilent Technologies, RRID: AB_10013382 ), and aspartoacylase (ASPA, 1:1000, kindly provided by Dr. M.A.
Article Title: NF-κB Activation Protects Oligodendrocytes against Inflammation
Article Snippet: 224 Immunohistochemical detection of CC1 (APC7, 1:50; EMD Biosciences, Gibbstown, NJ, 225 RRID:AB_2057371), myelin basic protein (MBP,1:1000; Sternberger Monoclonals, Berkeley, 226 CA, RRID:AB_10120129), CD3 (1:50; Santa Cruz Biotechnology, RRID:AB_627010), CD11b 227 11 (1:50; Millipore, Temecula, CA, RRID:AB_92930), the active form of p65 (1:50; Millipore, 228 RRID:AB_2178887), glial fibiralliry acidic protein (GFAP; 1:200; Agilent Technologies, Santa 229 Clara, CA, RRID:AB_10013382) and aspartoacylase (ASPA, 1:1000, kindly provided by Dr. 230 M.A.
Real-time Polymerase Chain Reaction:Article Title: The Integrated UPR and ERAD in Oligodendrocytes Maintain Myelin Thickness in Adults by Regulating Myelin Protein Translation
Article Snippet: Immunohistochemical detection of CC1 (APC7, 1:50; EMD Biosciences, Gibbstown, 194 NJ, RRID:AB_2057371), myelin basic protein (MBP, 1:1000; Sternberger Monoclonals, 195 Berkeley, CA, RRID:AB_10120129), aspartoacylase (ASPA, 1:1000, kindly provided by Dr. 196 M.A.
Article Title: Oligodendrocyte-specific ATF4 inactivation does not influence the development of EAE
Article Snippet: Immunohistochemistry (IHC) for CC1 (APC7, 1:50; EMD Biosciences, Gibbstown, NJ, RRID:AB_2057371), myelin basic protein (MBP, 1:1000; Sternberger Monoclonals, Berkeley, CA, RRID:AB_10120129), CD3 (1:50; Santa Cruz Biotechnology, RRID:AB_627010), CD11b (1:50; Millipore, Temecula, CA, RRID:AB_92930), NeuN (1:500, Millipore, RRID:AB_2298772), phosphorylated neuro-filament-H (SMI31, 1:1000, Sternberger Monoclonals, RRID:AB_509995), non-phosphorylated neuro-filament-H (SMI32, 1:1000, Covance, San Diego, CA, USA, RRID:AB_509997), CHOP (1:100, Santa Cruz Biotechnology, RRID: AB_783507), calbindin 2 (1:400, Sigma-Aldrich, RRID:AB_476894), and aspartoacylase (ASPA, 1:1000, kindly provided by Dr. M.A.
Article Title: NF-κB Activation Accounts for the Cytoprotective Effects of PERK Activation on Oligodendrocytes during EAE
Article Snippet: Immunohistochemical detection of CC1 (APC7, 207 1:50; EMD Biosciences, Gibbstown, NJ, RRID:AB_2057371), myelin basic protein 208 10 (MBP,1:1000; Sternberger Monoclonals, Berkeley, CA, RRID:AB_10120129), CD3 (1:50; 209 Santa Cruz Biotechnology, RRID:AB_627010), CD11b (1:50; Millipore, Temecula, CA, 210 RRID:AB_92930), the active form of p65 (1:50; Millipore, RRID:AB_2178887), aspartoacylase 211 (ASPA, 1:500; Millipore, Catalog: ABN1698), Flag (1:250; Sigma, RRID: AB_1957945), 212 SMI32 (1:1000; Covance, RRID: AB_509997), glial fibrillary acidic protein (GFAP, 1:1000; 213 Covance, RRID:AB_2314542), and A20/TNFAIP3 (1:100; Santa Cruz Biotechnology, Catalog: 214 sc-166692) were performed.
Article Title: An expanded polyglutamine in ATAXIN1 results in a loss-of-function that exacerbates severity of Multiple Sclerosis in an EAE mouse model.
Article Snippet: We used the following primary and secondary antibodies for immunohistochemical detection: CC1 (APC7, 1:50; Millipore, RRID: AB_2057371), myelin basic protein (MBP,1:1000; BioLegend, RRID: AB_2616694), CD3 (1:50; BioLegend, RRID: AB_312658), NeuN (1:500; Abcam, RRID: AB_10711040), glial fibrillary acidic protein (GFAP; 1:200; Agilent Technologies, RRID: AB_10013382), Iba1 (1:200; FUJIFILM Wako’s, RRID: AB_839504), and Neurofilament H (NF-H) (1:200; BioLegend, RRID: AB_32715852).
Article Title: Activating transcription factor 6α deficiency exacerbates oligodendrocyte death and myelin damage in immune-mediated demyelinating diseases
Article Snippet: Immunohistochemical detection of CC1 (APC7, 1:50; EMD Biosciences, Gibbstown, NJ, RRID:AB_2057371), myelin basic protein (MBP, 1:1000; Sternberger Monoclonals, Berkeley, CA, RRID:AB_10120129), CD3 (1:50; Santa Cruz Biotechnology, Dallas, Texas, RRID:AB_627010), CD11b (1:50; Millipore, RRID:AB_92930), immunoglobulin-heavy-chain-binding protein (BiP, 1:100; Santa Cruz Biotechnology, RRID:AB_631616), Glial fibrillary acidic protein (GFAP, 1:1000, Covance, Princeton, NJ, RRID:AB_2314542), Iba1 (1:300, Wako Pure Chemical Industries, Ltd, Osaka, Japan; RRID:AB_2665520), and aspartoacylase (ASPA, 1:1000, kindly provided by Dr. M.A.
Article Title: NF-κB Activation Protects Oligodendrocytes against Inflammation
Article Snippet: Immunohistochemical detection of CC1 (APC7, 1:50; EMD Biosciences, RRID: AB_2057371 ), myelin basic protein (MBP,1:1000; Sternberger Monoclonals, RRID: AB_10120129 ), CD3 (1:50; Santa Cruz Biotechnology, RRID: AB_627010 ), CD11b (1:50; Millipore, RRID: AB_92930 ), the active form of p65 (1:50; Millipore, RRID: AB_2178887 ), glial fibrillary acidic protein (GFAP; 1:200; Agilent Technologies, RRID: AB_10013382 ), and aspartoacylase (ASPA, 1:1000, kindly provided by Dr. M.A.
Article Title: NF-κB Activation Protects Oligodendrocytes against Inflammation
Article Snippet: 224 Immunohistochemical detection of CC1 (APC7, 1:50; EMD Biosciences, Gibbstown, NJ, 225 RRID:AB_2057371), myelin basic protein (MBP,1:1000; Sternberger Monoclonals, Berkeley, 226 CA, RRID:AB_10120129), CD3 (1:50; Santa Cruz Biotechnology, RRID:AB_627010), CD11b 227 11 (1:50; Millipore, Temecula, CA, RRID:AB_92930), the active form of p65 (1:50; Millipore, 228 RRID:AB_2178887), glial fibiralliry acidic protein (GFAP; 1:200; Agilent Technologies, Santa 229 Clara, CA, RRID:AB_10013382) and aspartoacylase (ASPA, 1:1000, kindly provided by Dr. 230 M.A.
Immunohistochemistry:Article Title: The Integrated UPR and ERAD in Oligodendrocytes Maintain Myelin Thickness in Adults by Regulating Myelin Protein Translation
Article Snippet: Immunohistochemical detection of CC1 (APC7, 1:50; EMD Biosciences, Gibbstown, 194 NJ, RRID:AB_2057371), myelin basic protein (MBP, 1:1000; Sternberger Monoclonals, 195 Berkeley, CA, RRID:AB_10120129), aspartoacylase (ASPA, 1:1000, kindly provided by Dr. 196 M.A.
Article Title: Oligodendrocyte-specific ATF4 inactivation does not influence the development of EAE
Article Snippet: Immunohistochemistry (IHC) for CC1 (APC7, 1:50; EMD Biosciences, Gibbstown, NJ, RRID:AB_2057371), myelin basic protein (MBP, 1:1000; Sternberger Monoclonals, Berkeley, CA, RRID:AB_10120129), CD3 (1:50; Santa Cruz Biotechnology, RRID:AB_627010), CD11b (1:50; Millipore, Temecula, CA, RRID:AB_92930), NeuN (1:500, Millipore, RRID:AB_2298772), phosphorylated neuro-filament-H (SMI31, 1:1000, Sternberger Monoclonals, RRID:AB_509995), non-phosphorylated neuro-filament-H (SMI32, 1:1000, Covance, San Diego, CA, USA, RRID:AB_509997), CHOP (1:100, Santa Cruz Biotechnology, RRID: AB_783507), calbindin 2 (1:400, Sigma-Aldrich, RRID:AB_476894), and aspartoacylase (ASPA, 1:1000, kindly provided by Dr. M.A.
Article Title: NF-κB Activation Accounts for the Cytoprotective Effects of PERK Activation on Oligodendrocytes during EAE
Article Snippet: Immunohistochemical detection of CC1 (APC7, 207 1:50; EMD Biosciences, Gibbstown, NJ, RRID:AB_2057371), myelin basic protein 208 10 (MBP,1:1000; Sternberger Monoclonals, Berkeley, CA, RRID:AB_10120129), CD3 (1:50; 209 Santa Cruz Biotechnology, RRID:AB_627010), CD11b (1:50; Millipore, Temecula, CA, 210 RRID:AB_92930), the active form of p65 (1:50; Millipore, RRID:AB_2178887), aspartoacylase 211 (ASPA, 1:500; Millipore, Catalog: ABN1698), Flag (1:250; Sigma, RRID: AB_1957945), 212 SMI32 (1:1000; Covance, RRID: AB_509997), glial fibrillary acidic protein (GFAP, 1:1000; 213 Covance, RRID:AB_2314542), and A20/TNFAIP3 (1:100; Santa Cruz Biotechnology, Catalog: 214 sc-166692) were performed.
Article Title: An expanded polyglutamine in ATAXIN1 results in a loss-of-function that exacerbates severity of Multiple Sclerosis in an EAE mouse model.
Article Snippet: We used the following primary and secondary antibodies for immunohistochemical detection: CC1 (APC7, 1:50; Millipore, RRID: AB_2057371), myelin basic protein (MBP,1:1000; BioLegend, RRID: AB_2616694), CD3 (1:50; BioLegend, RRID: AB_312658), NeuN (1:500; Abcam, RRID: AB_10711040), glial fibrillary acidic protein (GFAP; 1:200; Agilent Technologies, RRID: AB_10013382), Iba1 (1:200; FUJIFILM Wako’s, RRID: AB_839504), and Neurofilament H (NF-H) (1:200; BioLegend, RRID: AB_32715852).
Article Title: Activating transcription factor 6α deficiency exacerbates oligodendrocyte death and myelin damage in immune-mediated demyelinating diseases
Article Snippet: Immunohistochemical detection of CC1 (APC7, 1:50; EMD Biosciences, Gibbstown, NJ, RRID:AB_2057371), myelin basic protein (MBP, 1:1000; Sternberger Monoclonals, Berkeley, CA, RRID:AB_10120129), CD3 (1:50; Santa Cruz Biotechnology, Dallas, Texas, RRID:AB_627010), CD11b (1:50; Millipore, RRID:AB_92930), immunoglobulin-heavy-chain-binding protein (BiP, 1:100; Santa Cruz Biotechnology, RRID:AB_631616), Glial fibrillary acidic protein (GFAP, 1:1000, Covance, Princeton, NJ, RRID:AB_2314542), Iba1 (1:300, Wako Pure Chemical Industries, Ltd, Osaka, Japan; RRID:AB_2665520), and aspartoacylase (ASPA, 1:1000, kindly provided by Dr. M.A.
Article Title: NF-κB Activation Protects Oligodendrocytes against Inflammation
Article Snippet: Immunohistochemical detection of CC1 (APC7, 1:50; EMD Biosciences, RRID: AB_2057371 ), myelin basic protein (MBP,1:1000; Sternberger Monoclonals, RRID: AB_10120129 ), CD3 (1:50; Santa Cruz Biotechnology, RRID: AB_627010 ), CD11b (1:50; Millipore, RRID: AB_92930 ), the active form of p65 (1:50; Millipore, RRID: AB_2178887 ), glial fibrillary acidic protein (GFAP; 1:200; Agilent Technologies, RRID: AB_10013382 ), and aspartoacylase (ASPA, 1:1000, kindly provided by Dr. M.A.
Article Title: NF-κB Activation Protects Oligodendrocytes against Inflammation
Article Snippet: 224 Immunohistochemical detection of CC1 (APC7, 1:50; EMD Biosciences, Gibbstown, NJ, 225 RRID:AB_2057371), myelin basic protein (MBP,1:1000; Sternberger Monoclonals, Berkeley, 226 CA, RRID:AB_10120129), CD3 (1:50; Santa Cruz Biotechnology, RRID:AB_627010), CD11b 227 11 (1:50; Millipore, Temecula, CA, RRID:AB_92930), the active form of p65 (1:50; Millipore, 228 RRID:AB_2178887), glial fibiralliry acidic protein (GFAP; 1:200; Agilent Technologies, Santa 229 Clara, CA, RRID:AB_10013382) and aspartoacylase (ASPA, 1:1000, kindly provided by Dr. 230 M.A.
Knock-Out:Article Title: The Integrated UPR and ERAD in Oligodendrocytes Maintain Myelin Thickness in Adults by Regulating Myelin Protein Translation
Article Snippet: Immunohistochemical detection of CC1 (APC7, 1:50; EMD Biosciences, Gibbstown, 194 NJ, RRID:AB_2057371), myelin basic protein (MBP, 1:1000; Sternberger Monoclonals, 195 Berkeley, CA, RRID:AB_10120129), aspartoacylase (ASPA, 1:1000, kindly provided by Dr. 196 M.A.
Article Title: Oligodendrocyte-specific ATF4 inactivation does not influence the development of EAE
Article Snippet: Immunohistochemistry (IHC) for CC1 (APC7, 1:50; EMD Biosciences, Gibbstown, NJ, RRID:AB_2057371), myelin basic protein (MBP, 1:1000; Sternberger Monoclonals, Berkeley, CA, RRID:AB_10120129), CD3 (1:50; Santa Cruz Biotechnology, RRID:AB_627010), CD11b (1:50; Millipore, Temecula, CA, RRID:AB_92930), NeuN (1:500, Millipore, RRID:AB_2298772), phosphorylated neuro-filament-H (SMI31, 1:1000, Sternberger Monoclonals, RRID:AB_509995), non-phosphorylated neuro-filament-H (SMI32, 1:1000, Covance, San Diego, CA, USA, RRID:AB_509997), CHOP (1:100, Santa Cruz Biotechnology, RRID: AB_783507), calbindin 2 (1:400, Sigma-Aldrich, RRID:AB_476894), and aspartoacylase (ASPA, 1:1000, kindly provided by Dr. M.A.
Article Title: NF-κB Activation Accounts for the Cytoprotective Effects of PERK Activation on Oligodendrocytes during EAE
Article Snippet: Immunohistochemical detection of CC1 (APC7, 207 1:50; EMD Biosciences, Gibbstown, NJ, RRID:AB_2057371), myelin basic protein 208 10 (MBP,1:1000; Sternberger Monoclonals, Berkeley, CA, RRID:AB_10120129), CD3 (1:50; 209 Santa Cruz Biotechnology, RRID:AB_627010), CD11b (1:50; Millipore, Temecula, CA, 210 RRID:AB_92930), the active form of p65 (1:50; Millipore, RRID:AB_2178887), aspartoacylase 211 (ASPA, 1:500; Millipore, Catalog: ABN1698), Flag (1:250; Sigma, RRID: AB_1957945), 212 SMI32 (1:1000; Covance, RRID: AB_509997), glial fibrillary acidic protein (GFAP, 1:1000; 213 Covance, RRID:AB_2314542), and A20/TNFAIP3 (1:100; Santa Cruz Biotechnology, Catalog: 214 sc-166692) were performed.
Article Title: An expanded polyglutamine in ATAXIN1 results in a loss-of-function that exacerbates severity of Multiple Sclerosis in an EAE mouse model.
Article Snippet: We used the following primary and secondary antibodies for immunohistochemical detection: CC1 (APC7, 1:50; Millipore, RRID: AB_2057371), myelin basic protein (MBP,1:1000; BioLegend, RRID: AB_2616694), CD3 (1:50; BioLegend, RRID: AB_312658), NeuN (1:500; Abcam, RRID: AB_10711040), glial fibrillary acidic protein (GFAP; 1:200; Agilent Technologies, RRID: AB_10013382), Iba1 (1:200; FUJIFILM Wako’s, RRID: AB_839504), and Neurofilament H (NF-H) (1:200; BioLegend, RRID: AB_32715852).
Article Title: Activating transcription factor 6α deficiency exacerbates oligodendrocyte death and myelin damage in immune-mediated demyelinating diseases
Article Snippet: Immunohistochemical detection of CC1 (APC7, 1:50; EMD Biosciences, Gibbstown, NJ, RRID:AB_2057371), myelin basic protein (MBP, 1:1000; Sternberger Monoclonals, Berkeley, CA, RRID:AB_10120129), CD3 (1:50; Santa Cruz Biotechnology, Dallas, Texas, RRID:AB_627010), CD11b (1:50; Millipore, RRID:AB_92930), immunoglobulin-heavy-chain-binding protein (BiP, 1:100; Santa Cruz Biotechnology, RRID:AB_631616), Glial fibrillary acidic protein (GFAP, 1:1000, Covance, Princeton, NJ, RRID:AB_2314542), Iba1 (1:300, Wako Pure Chemical Industries, Ltd, Osaka, Japan; RRID:AB_2665520), and aspartoacylase (ASPA, 1:1000, kindly provided by Dr. M.A.
Article Title: NF-κB Activation Protects Oligodendrocytes against Inflammation
Article Snippet: Immunohistochemical detection of CC1 (APC7, 1:50; EMD Biosciences, RRID: AB_2057371 ), myelin basic protein (MBP,1:1000; Sternberger Monoclonals, RRID: AB_10120129 ), CD3 (1:50; Santa Cruz Biotechnology, RRID: AB_627010 ), CD11b (1:50; Millipore, RRID: AB_92930 ), the active form of p65 (1:50; Millipore, RRID: AB_2178887 ), glial fibrillary acidic protein (GFAP; 1:200; Agilent Technologies, RRID: AB_10013382 ), and aspartoacylase (ASPA, 1:1000, kindly provided by Dr. M.A.
Article Title: NF-κB Activation Protects Oligodendrocytes against Inflammation
Article Snippet: 224 Immunohistochemical detection of CC1 (APC7, 1:50; EMD Biosciences, Gibbstown, NJ, 225 RRID:AB_2057371), myelin basic protein (MBP,1:1000; Sternberger Monoclonals, Berkeley, 226 CA, RRID:AB_10120129), CD3 (1:50; Santa Cruz Biotechnology, RRID:AB_627010), CD11b 227 11 (1:50; Millipore, Temecula, CA, RRID:AB_92930), the active form of p65 (1:50; Millipore, 228 RRID:AB_2178887), glial fibiralliry acidic protein (GFAP; 1:200; Agilent Technologies, Santa 229 Clara, CA, RRID:AB_10013382) and aspartoacylase (ASPA, 1:1000, kindly provided by Dr. 230 M.A.
Expressing:Article Title: The Integrated UPR and ERAD in Oligodendrocytes Maintain Myelin Thickness in Adults by Regulating Myelin Protein Translation
Article Snippet: Immunohistochemical detection of CC1 (APC7, 1:50; EMD Biosciences, Gibbstown, 194 NJ, RRID:AB_2057371), myelin basic protein (MBP, 1:1000; Sternberger Monoclonals, 195 Berkeley, CA, RRID:AB_10120129), aspartoacylase (ASPA, 1:1000, kindly provided by Dr. 196 M.A.
Article Title: Oligodendrocyte-specific ATF4 inactivation does not influence the development of EAE
Article Snippet: Immunohistochemistry (IHC) for CC1 (APC7, 1:50; EMD Biosciences, Gibbstown, NJ, RRID:AB_2057371), myelin basic protein (MBP, 1:1000; Sternberger Monoclonals, Berkeley, CA, RRID:AB_10120129), CD3 (1:50; Santa Cruz Biotechnology, RRID:AB_627010), CD11b (1:50; Millipore, Temecula, CA, RRID:AB_92930), NeuN (1:500, Millipore, RRID:AB_2298772), phosphorylated neuro-filament-H (SMI31, 1:1000, Sternberger Monoclonals, RRID:AB_509995), non-phosphorylated neuro-filament-H (SMI32, 1:1000, Covance, San Diego, CA, USA, RRID:AB_509997), CHOP (1:100, Santa Cruz Biotechnology, RRID: AB_783507), calbindin 2 (1:400, Sigma-Aldrich, RRID:AB_476894), and aspartoacylase (ASPA, 1:1000, kindly provided by Dr. M.A.
Article Title: NF-κB Activation Accounts for the Cytoprotective Effects of PERK Activation on Oligodendrocytes during EAE
Article Snippet: Immunohistochemical detection of CC1 (APC7, 207 1:50; EMD Biosciences, Gibbstown, NJ, RRID:AB_2057371), myelin basic protein 208 10 (MBP,1:1000; Sternberger Monoclonals, Berkeley, CA, RRID:AB_10120129), CD3 (1:50; 209 Santa Cruz Biotechnology, RRID:AB_627010), CD11b (1:50; Millipore, Temecula, CA, 210 RRID:AB_92930), the active form of p65 (1:50; Millipore, RRID:AB_2178887), aspartoacylase 211 (ASPA, 1:500; Millipore, Catalog: ABN1698), Flag (1:250; Sigma, RRID: AB_1957945), 212 SMI32 (1:1000; Covance, RRID: AB_509997), glial fibrillary acidic protein (GFAP, 1:1000; 213 Covance, RRID:AB_2314542), and A20/TNFAIP3 (1:100; Santa Cruz Biotechnology, Catalog: 214 sc-166692) were performed.
Article Title: An expanded polyglutamine in ATAXIN1 results in a loss-of-function that exacerbates severity of Multiple Sclerosis in an EAE mouse model.
Article Snippet: We used the following primary and secondary antibodies for immunohistochemical detection: CC1 (APC7, 1:50; Millipore, RRID: AB_2057371), myelin basic protein (MBP,1:1000; BioLegend, RRID: AB_2616694), CD3 (1:50; BioLegend, RRID: AB_312658), NeuN (1:500; Abcam, RRID: AB_10711040), glial fibrillary acidic protein (GFAP; 1:200; Agilent Technologies, RRID: AB_10013382), Iba1 (1:200; FUJIFILM Wako’s, RRID: AB_839504), and Neurofilament H (NF-H) (1:200; BioLegend, RRID: AB_32715852).
Article Title: Activating transcription factor 6α deficiency exacerbates oligodendrocyte death and myelin damage in immune-mediated demyelinating diseases
Article Snippet: Immunohistochemical detection of CC1 (APC7, 1:50; EMD Biosciences, Gibbstown, NJ, RRID:AB_2057371), myelin basic protein (MBP, 1:1000; Sternberger Monoclonals, Berkeley, CA, RRID:AB_10120129), CD3 (1:50; Santa Cruz Biotechnology, Dallas, Texas, RRID:AB_627010), CD11b (1:50; Millipore, RRID:AB_92930), immunoglobulin-heavy-chain-binding protein (BiP, 1:100; Santa Cruz Biotechnology, RRID:AB_631616), Glial fibrillary acidic protein (GFAP, 1:1000, Covance, Princeton, NJ, RRID:AB_2314542), Iba1 (1:300, Wako Pure Chemical Industries, Ltd, Osaka, Japan; RRID:AB_2665520), and aspartoacylase (ASPA, 1:1000, kindly provided by Dr. M.A.
Article Title: NF-κB Activation Protects Oligodendrocytes against Inflammation
Article Snippet: Immunohistochemical detection of CC1 (APC7, 1:50; EMD Biosciences, RRID: AB_2057371 ), myelin basic protein (MBP,1:1000; Sternberger Monoclonals, RRID: AB_10120129 ), CD3 (1:50; Santa Cruz Biotechnology, RRID: AB_627010 ), CD11b (1:50; Millipore, RRID: AB_92930 ), the active form of p65 (1:50; Millipore, RRID: AB_2178887 ), glial fibrillary acidic protein (GFAP; 1:200; Agilent Technologies, RRID: AB_10013382 ), and aspartoacylase (ASPA, 1:1000, kindly provided by Dr. M.A.
Article Title: NF-κB Activation Protects Oligodendrocytes against Inflammation
Article Snippet: 224 Immunohistochemical detection of CC1 (APC7, 1:50; EMD Biosciences, Gibbstown, NJ, 225 RRID:AB_2057371), myelin basic protein (MBP,1:1000; Sternberger Monoclonals, Berkeley, 226 CA, RRID:AB_10120129), CD3 (1:50; Santa Cruz Biotechnology, RRID:AB_627010), CD11b 227 11 (1:50; Millipore, Temecula, CA, RRID:AB_92930), the active form of p65 (1:50; Millipore, 228 RRID:AB_2178887), glial fibiralliry acidic protein (GFAP; 1:200; Agilent Technologies, Santa 229 Clara, CA, RRID:AB_10013382) and aspartoacylase (ASPA, 1:1000, kindly provided by Dr. 230 M.A.
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Millipore is a verified supplier 